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Purification, N-terminal sequence and structural characterization of a toxic protein from the Indian scorpion venom
1Department of Life Sciences, University of Bombay, India.
Abstract:
A toxic protein component (Bt-II) was isolated from the venom of the Indian scorpion, Buthus tamulus, by ion-exchange chromatography on Biorex-70 and reverse phase high pressure liquid chromatography (RP-HPLC). This 7800 mol. wt protein was found to have an LD50 value of 2.25 mg/kg body weight in mice by subcutaneous injection. Amino acid composition of the protein revealed the presence of aromatic and basic amino acids; the N-terminal amino acid sequence was also determined. Structural characterization of Bt-II, based on circular dichroic and fluorescent quenching studies, has also been reported.