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Human islet isolation in 104 consecutive cases. Factors affecting isolation success
P Y Benhamou1, P C Watt, Y Mullen
1Department of Surgery, UCLA School of Medicine 90024-7036.
Transplantation
|June 27, 1994
Summary
Optimizing islet isolation for transplantation requires careful donor selection and standardized techniques. Key factors influencing islet viability include donor blood glucose and the specific collagenase used during processing.
Area of Science:
- Endocrinology
- Transplantation Immunology
- Cell Biology
Background:
- Successful islet transplantation is crucial for treating type 1 diabetes.
- Obtaining sufficient numbers of viable islets is a major challenge.
- Donor factors and isolation protocols significantly impact islet yield and viability.
Purpose of the Study:
- To analyze factors influencing islet isolation outcomes.
- To identify predictors of viable islet recovery and overall islet yield.
- To improve islet isolation protocols for transplantation.
Main Methods:
- Retrospective analysis of 104 cadaveric human pancreata processed for islet isolation.
- Standardized islet isolation method by a single team.
- Evaluation of endpoints: viable islet recovery and islet yield after 2 days of culture.
Main Results:
- Viable islets were recovered in 61% of cases.
- Lower donor blood glucose and lack of significant medical history correlated with better viability.
- Collagenase lot and cold ischemia time significantly affected viable islet recovery.
- Multivariate analysis identified blood glucose and collagenase lot as key risk factors for viability.
Conclusions:
- Donor blood glucose, collagenase lot, and procurement team are critical factors for successful islet isolation.
- Further research should focus on optimizing these donor and technical aspects to enhance islet yield and viability for transplantation.
- Improved islet isolation protocols are essential for advancing islet transplantation therapies.