Fractured carbon fiber-based biosensor for glucose

Area of Science:

• Electrochemistry
• Biosensors
• Materials Science

Background:

• Conventional glucose sensors face limitations in sensitivity and stability.
• DuPont E120 fibers possess unique fracturing properties upon anodic pretreatment.
• Increased electrochemical surface area is crucial for enhanced sensor performance.

Purpose of the Study:

• To design and construct a novel glucose microsensor utilizing fractured E120 fibers.
• To leverage the increased electrochemical surface area for improved glucose detection.
• To evaluate the performance and stability of the developed glucose microsensor.

Main Methods:

• Severe anodic pretreatment of DuPont E120 fibers to induce extensive fracturing.
• Platinization of fractured fibers to enhance hydrogen peroxide detection.
• Immobilization of glucose oxidase in polypyrrole onto the fiber surface.
• Amperometric detection in flow injection analysis.

Main Results:

• Fracturing significantly increased the electrochemical surface area of the fibers.
• The microsensor exhibited a linear response to glucose concentration up to 10 mM.
• An apparent Michaelis-Menten constant (Km') of approximately 20 mM was observed.
• The sensor demonstrated stability for up to 2 months when stored dry at 4°C.

Conclusions:

• Fractured DuPont E120 fibers provide a promising platform for glucose microsensor development.
• The enhanced electrochemical surface area and enzyme immobilization contribute to sensitive detection.
• The developed microsensor offers good linearity, stability, and a practical detection range for glucose monitoring.