Lipoprotein-dependent unsaturated fatty acid transport and metabolism in cultured cells

Our results can be summarized as follows. LDL stimulates eicosanoid formation through delivery of substrate AA to enzymes of oxidative AA metabolism. The classical LDL receptor controls the effect of LDL AA on eicosanoid formation. LDL AA metabolism occurs in several cell types of mesenchymal and epithelial origin and generates the formation of distinct eicosanoid patterns in a tissue-specific way. LDL inhibits the PGH synthase, and the LDL-dependent inhibition of the enzyme resembles the inhibition by unesterified AA. The LDL AA pathway does appear to couple directly to the PGH synthase reaction, but it does not appear to couple directly to the 5-lipoxygenase reaction. We expect that a more complete characterization of the LDL unsaturated fatty acid pathway in different tissues will yield additional information on the biochemistry of both lipoproteins and AA metabolism.