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A common deletion in two gamma ray induced rat pulmonary tumor cell lines
P Van Klaveren1, J De Bruijne, H Van der Winden
1Department of Experimental Dosimetry, TNO, Rijswijk, The Netherlands.
Anticancer Research
|March 1, 1994
Abstract:
Subtraction hybridization was performed on normal WAG/Rij rat DNA with DNA from a syngeneic Ir-192 induced pulmonary tumor cell line L37. The residual DNA was amplified by means of sequence-independent PCR. This procedure yielded a sequence, of which multiple copies are present in normal rat DNA. In the tumor line L37 two restriction fragments hybridizing with this repeat sequence are lacking. In another Ir-192 induced pulmonary tumor line, L33, one of these fragments was also lacking. This indicates a common deletion in the two tumor lines.