Multiple NF-I-site binding factors associated with different cell-types of human lung carcinomas

Nuclear extracts from human lung adenocarcinoma and squamous carcinoma cells were found to form two types of DNA-protein complexes with a consensus nuclear factor I (NF-I)-binding site. Three NF-I-site-binding cofactors (NCF90, NCFIIO and NCF120) were found to be differentially expressed in these two cell-types of human lung carcinomas. These data suggest that the NF-I-binding enhancer element with its differentially expressed NF-I cofactors may play an important role in the differentiation of neoplastic human bronchial epithelium.