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Updated: Jul 11, 2026
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High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli
Published on: August 13, 2011
Purification and characterization of the CelB endoglucanase from Streptomyces lividans 66 and DNA sequence of the
S Wittmann1, F Shareck, D Kluepfel
1Centre de Recherche en Microbiologie Appliquée, Institut Armand-Frappier, Université du Québec, Laval-des-Rapides, Canada.
Applied and Environmental Microbiology
|May 1, 1994
Abstract:
The endoglucanase CelB isolated from culture filtrates of Streptomyces lividans IAF9 has an M(r) of 36,000. With carboxymethyl cellulose as the substrate, the Vmax and Km values are 110 IU/mg of enzyme and 1.3 mg/ml, respectively. Comparison of primary amino acid sequences classifies CelB in the H family of cellulases.
