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A novel serine proteinase-like sequence from human brain
1Department of Biochemistry, University of Basel, Switzerland.
Biochimica Et Biophysica Acta
|June 21, 1994
Abstract:
From Alzheimer's visual cortex mRNA, a novel cDNA sequence with homology to the carboxy-terminal two-thirds of known serine proteinases was isolated (43% identity with human trypsinogen II). The corresponding mRNA has a size of approximately 8 kb and is expressed in low amounts in all human tissues tested.
Insights
Researchers identified a novel serine proteinase-like cDNA sequence in Alzheimer
Area of Science:
- Neuroscience
- Molecular Biology
- Biochemistry
Background:
- Alzheimer's disease is a neurodegenerative disorder.
- The visual cortex is affected in Alzheimer's disease.
- Serine proteinases play diverse biological roles.
Purpose of the Study:
- To identify novel genes or sequences in the Alzheimer's disease visual cortex.
- To characterize a newly isolated cDNA sequence.
Main Methods:
- Messenger RNA (mRNA) isolation from Alzheimer's disease visual cortex.
- cDNA sequencing and homology analysis.
- Northern blot analysis for mRNA size and tissue expression.
Main Results:
- A novel cDNA sequence was isolated, showing homology to the carboxy-terminal region of serine proteinases.
- The sequence exhibited 43% identity to human trypsinogen II.
- The corresponding mRNA is approximately 8 kb and expressed at low levels in all tested human tissues.
Conclusions:
- A novel serine proteinase-related sequence is present in the human brain.
- The low-level ubiquitous expression suggests a potential role in basic cellular functions.
- Further research is needed to determine the function and significance of this novel sequence in Alzheimer's disease and other tissues.