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Expression of the platelet-derived growth factor beta receptor during organogenesis and tissue differentiation in the
E Shinbrot1, K G Peters, L T Williams
1Cardiovascular Research Institute, University of California-San Francisco 94143.
Abstract:
In this study we used in situ hybridization to localize expression of the platelet-derived growth factor beta (PDGF beta) receptor mRNA during organogenesis in the mouse embryo (E 9.5-16.5). Expression was first seen in periaortic mesenchyme (E 9.5-10.5). Later (E 12.5-E 16.5), the receptor was expressed in the mesenchymal component of many developing tissues and organs, particularly derivatives of the primitive gut. The expression was exceptionally high in mesenchyme directly supporting an epithelium, typical of many developing organs such as the trachea and intestine. However, as the mesenchyme differentiated into smooth muscle, PDGF beta receptor mRNA was no longer detected. The expression of the PDGF beta receptor mRNA in mesenchymal components of developing organs, along with its absence in epithelial tissues, indicates that it may play a role in mesenchymal-epithelial interactions during organ development. Somewhat unexpectedly, the PDGF beta receptor was highly expressed in the endothelium of small blood vessels and vascular structures such as the hyaloid plexus and choroid plexus. In large blood vessels, PDGF beta receptor mRNA was found in the mesenchyme surrounding the endothelium. This suggests that the PDGF beta receptor is involved in growth and development of blood vessels.
Insights
Platelet-derived growth factor beta (PDGF beta) receptor mRNA is crucial for mouse embryo organogenesis. Its expression in mesenchyme and blood vessel endothelium highlights roles in development and mesenchymal-epithelial interactions.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genetics
Background:
- The platelet-derived growth factor beta (PDGF beta) receptor plays a role in cell growth and differentiation.
- Understanding its expression patterns during embryonic development is key to deciphering its functions.
Purpose of the Study:
- To investigate the spatiotemporal expression of PDGF beta receptor mRNA during mouse embryonic organogenesis.
- To elucidate the potential roles of PDGF beta receptor in mesenchymal-epithelial interactions and vascular development.
Main Methods:
- In situ hybridization was employed to localize PDGF beta receptor mRNA expression in mouse embryos from embryonic day 9.5 to 16.5.
- Detailed analysis of expression patterns in various developing tissues and organs.
Main Results:
- PDGF beta receptor mRNA was detected in periaortic mesenchyme early in development (E 9.5-10.5).
- Later, expression was observed in the mesenchymal component of numerous organs, particularly those derived from the primitive gut, and was high in mesenchyme supporting epithelia.
- Unexpectedly high expression was found in the endothelium of small blood vessels and vascular structures, with expression in the surrounding mesenchyme of large vessels.
- Expression diminished as mesenchyme differentiated into smooth muscle.
Conclusions:
- PDGF beta receptor mRNA expression in mesenchyme suggests a role in mesenchymal-epithelial interactions during organ development.
- Its presence in vascular endothelium and surrounding mesenchyme indicates involvement in blood vessel growth and development.
- The findings provide insights into the complex roles of PDGF beta signaling in embryonic development.