Related Experiment Videos
Radiation-induction of micronuclei in human peripheral blood lymphocytes: effect of freezing
J S Prosser1, B E Izard, M A Wallace
1Biomedicine and Health Program, Australian Nuclear Science and Technology Organisation, Menai, NSW.
Cytobios
|January 1, 1994
Abstract:
Lymphocytes in peripheral blood samples from three donors were irradiated at doses of 0, 1 and 2 Gy with cobalt-60 gamma rays. Whole blood cultures were established immediately after irradiation. Cultures of Ficoll-Paque separated lymphocytes were set up immediately after irradiation, or 3 weeks after storage in liquid nitrogen at -196 degrees C. The frequency of micronuclei was measured in binucleate cytokinesis-blocked cells and the different treatment groups were compared. No clastogenic effect of the lymphocyte separation technique, or of subsequent freezing in liquid nitrogen, was observed.