Effect of cis-diamminedichloroplatinum(II) and other platinum compounds on the RNA polymerase reaction

The in vitro and in vivo effects of cis-diamminedichloroplatinum(II) (cis-DDP), transdiamminedichloroplatinum(II), spermine-platinum(II) complex and K2PtCl4 on the RNA polymerase reaction in isolated nuclei from Ehrlich ascites tumour cells were investigated. Incubation of nuclei in the presence of platinum compounds did not substantially influence the RNA polymerase reaction. The results from the in vivo experiments (i.e. after i.p. injection of mice with platinum compounds before nuclei isolation) showed that the compounds displayed distinct inhibitory effects on the reaction which was most pronounced with K2PtCl4 and cis-DDP.