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Liver injury and alterations of hepatic microsomal monooxygenase system due to dimethylformamide (DMF) in rats
K Imazu1, K Fujishiro, N Inoue
1NTT Kitakyushu Hospital, Kitakyushu.
Abstract:
The effects of repeated exposure to N, N-dimethylformamide (DMF) on the liver and the hepatic microsomal monooxygenase system were investigated. DMF was administered to Wistar male rats by subcutaneous (s.c.) injection at 0.5 ml/kg body weight daily for 1-17 days. Macroscopically, mild liver swelling was observed and liver weights significantly increased after 3 days of exposure to DMF respectively. Hematological changes were not observed. In exposed rats, glutamic oxaloacetic transaminase and glutamic pyruvic transaminase significantly increased after 3 and 7 days of exposure to DMF. After 17 days of exposure to DMF, transaminases decreased compared to the 7 days. Hepatic microsomal cytochrome P-450 tended to decrease after 3 days of exposure DMF and decreased by 34% after 7 days and 25% after 17 days. Aminopyrine N-demethylase activity depressed significantly while aniline hydroxylase activity showed no change after 3 days of exposure to DMF. These results indicate that DMF alters the hepatic microsomal monooxygenase system. These findings may greatly contribute to the elucidation of the pathogenesis of DMF hepatotoxicity.
Insights
Repeated N, N-dimethylformamide (DMF) exposure in rats caused liver swelling and elevated liver enzymes. Hepatic microsomal cytochrome P-450 and enzyme activities were altered, indicating DMF
Area of Science:
- Toxicology
- Hepatology
- Biochemistry
Background:
- N, N-dimethylformamide (DMF) is an industrial solvent with potential toxic effects.
- Understanding DMF's impact on liver function and metabolism is crucial for occupational safety.
Purpose of the Study:
- To investigate the effects of repeated N, N-dimethylformamide (DMF) exposure on rat liver and hepatic microsomal monooxygenase system.
- To elucidate the pathogenesis of DMF-induced hepatotoxicity.
Main Methods:
- Wistar male rats were administered DMF subcutaneously (0.5 ml/kg) daily for 1-17 days.
- Liver weights, hematology, serum transaminases (GOT, GPT), and hepatic microsomal enzyme activities (cytochrome P-450, aminopyrine N-demethylase, aniline hydroxylase) were assessed.
Main Results:
- Significant liver swelling and increased liver weights were observed after 3 days of DMF exposure.
- Elevated serum transaminases (GOT, GPT) were noted after 3 and 7 days, decreasing by day 17.
- Hepatic microsomal cytochrome P-450 levels decreased, and aminopyrine N-demethylase activity was significantly depressed, while aniline hydroxylase activity remained unchanged.
Conclusions:
- Repeated DMF exposure alters the hepatic microsomal monooxygenase system in rats.
- These alterations contribute to the understanding of DMF-induced liver damage.