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Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
Published on: November 12, 2012
Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2
N K Menon1, C Y Chatelus, M Dervartanian
1Department of Biochemistry, University of Georgia, Athens 30602.
Journal of Bacteriology
|July 1, 1994
Abstract:
The genes encoding the two structural subunits of Escherichia coli hydrogenase 2 (HYD2) have been cloned and sequenced. They occur in an operon (hyb) which contains seven open reading frames. An hyb deletion mutant (strain AP3) failed to grown on dihydrogen-fumarate medium and also produced very low levels of HYD1. All seven open reading frames are required for restoration of wild-type levels of active HYD2 in AP3. The hyb operon was mapped at 65 min on the E. coli chromosome.

