Effects of epoxyeicosatrienoic acids on 86Rb uptake in renal epithelial cells
R Staudinger1, B Escalante, M L Schwartzman
1Department of Pharmacology, Rockefeller University, New York, New York 10021.
Abstract:
Epoxyeicosatrienoic acids (EETs) are arachidonic acid metabolites formed endogenously via the cytochrome P450 pathway in rat, rabbit, and human kidney. We characterized the effects of the four regioisomeric EETs on ion transport in the renal epithelial cell line, LLC-PK1. Among the EETs, 14,15-EET was the most potent inhibitor of 86Rb uptake. Its effect was concentration-dependent (IC50 = 75 nM) and stereoselective to the 14S, 15R-EET. Experiments measuring 14,15-EET-induced 86Rb uptake inhibition in the presence of inhibitors of Na(+)-K(+)-ATPase activity (ouabain), Na(+)-K(+)-Cl- cotransporter (furosemide), and Na(+)-H+ exchanger (amiloride) suggested that 14,15-EET inhibits ion transport via an amiloride-sensitive mechanism. These results, together with previous reports demonstrating their endogenous production in the kidney, suggest an important role for EETs, specifically 14,15-EET, in the regulation of ion and water reabsorption in the kidney and implicate their function in renal pathophysiology.
More Related Videos
16:26Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport
Published on: June 28, 2014
06:32Development of Human Renal Tubular Epithelial Cell Primary Cultures in Monolayers and Three-Dimensional Conditions
Published on: June 13, 2025
Related Concept Videos
Drug Elimination by Renal Route: Tubular Secretion
Treatment for Pulmonary Arterial Hypertension: Endothelin Receptor Antagonists
ETs are synthesized through a complex sequence of enzymatic steps, primarily involving an enzyme referred to as endothelin-converting enzyme (ECE). Of...
Renal Corpuscle
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous capillaries...
Renal Drug Excretion: Tubular Reabsorption
Renal Drug Excretion: Tubular Secretion
