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Related Experiment Videos

Tumor necrosis factor-alpha expression in ischemic neurons

T Liu1, R K Clark, P C McDonnell

  • 1Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406.

Stroke
|July 1, 1994
PubMed
Summary
This summary is machine-generated.

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Tumor necrosis factor-alpha (TNF-alpha) mRNA and protein are elevated in the brain after experimental stroke, preceding leukocyte accumulation. This neuronal expression may worsen tissue damage and stroke risk.

Area of Science:

  • Neuroscience
  • Immunology
  • Pathology

Background:

  • Tumor necrosis factor-alpha (TNF-alpha) is a pro-inflammatory cytokine.
  • TNF-alpha promotes leukocyte adhesion to endothelial cells.
  • Leukocyte infiltration occurs in ischemic brain lesions.

Purpose of the Study:

  • To investigate TNF-alpha mRNA and peptide expression in the brain following experimental focal stroke.
  • To determine if TNF-alpha expression precedes leukocyte accumulation in ischemic brain tissue.

Main Methods:

  • Focal cerebral ischemia was induced in rats via permanent middle cerebral artery occlusion.
  • TNF-alpha mRNA and protein levels were measured in the ischemic and non-ischemic cerebral cortex.
  • The effect of exogenous TNF-alpha on leukocyte adherence was assessed.

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Main Results:

  • TNF-alpha mRNA levels increased significantly in the ischemic cortex within 1 hour, peaking at 12 hours.
  • TNF-alpha protein was detected in nerve fibers within the infarct and surrounding tissues.
  • Administering TNF-alpha to the cortex induced significant neutrophil adherence.

Conclusions:

  • Focal cerebral ischemia elevates TNF-alpha mRNA and protein in ischemic neurons.
  • Neuronal TNF-alpha expression may facilitate inflammatory cell infiltration.
  • This process could exacerbate tissue damage and increase stroke risk.