Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Serum response factor associated ETS proteins: ternary complex factors and PEA3-binding factor

S H Liu¹, S Y Ng

¹Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China.

Biochemical and Biophysical Research Communications

|June 30, 1994

Summary

A novel 60-kDa human ETS protein binds DNA independently and also interacts with Serum Response Factor (SRF). This protein recognizes sequences in promoters, suggesting a role in gene regulation via distinct ETS oncoproteins.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Impact of cumulative atherogenic index of plasma exposure on the risk of new-onset cardiovascular disease in middle-aged and young populations].

Zhonghua xin xue guan bing za zhi·2026

Same author

[Correlations of foveal avascular zone area, acircularity index and vision before and after surgery for idiopathic macular epiretinal membrane].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2026

Same author

[Effects of allergens on the expression levels of interleukin 18, interleukin 18 binding protein a and interleukin 18 receptor α in the blood monocyte subtypes of patients with allergic asthma].

Zhonghua nei ke za zhi·2025

Same author

[Effects of acellular allogeneic dermis combined with autologous split-thickness skin grafts in repairing deep burn wounds in head, face, neck, and torso in children].

Zhonghua shao shang yu chuang mian xiu fu za zhi·2025

Same author

[Comparison of positive criteria for ATS/ERS bronchodiastolic tests in 2005 and 2021 with different degree of airway obstruction].

Zhonghua yi xue za zhi·2025

Same author

[A case of cirrhosis combined with myeloproliferative neoplasms].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2024

Area of Science:

Molecular Biology
Transcription Factor Research
Oncogene Signaling

Background:

The c-fos promoter contains an ETS protein binding motif (CAGGAT) adjacent to the Serum Response Element (SRE).
ETS proteins can form ternary complexes with Serum Response Factor (SRF) at the SRE, a key mechanism in gene regulation.
Similar motifs are found in promoters of immediate early zinc finger proteins, but not all can bind Ternary Complex Factor (TCF).

Purpose of the Study:

To identify and characterize a novel ETS protein capable of binding TCF-recognized sequences.
To investigate the interaction of this novel ETS protein with SRF.
To elucidate the role of distinct ETS oncoproteins in gene regulation.

Main Methods:

Purification of a 60-kDa human ETS protein.

Related Experiment Videos

DNA-binding assays to assess binding to TCF-recognized sequences.

In vitro binding assays to study interactions between the purified ETS protein and SRF.

Main Results:

A 60-kDa human ETS protein was purified that binds the TCF-recognized DNA sequence.
This ETS protein demonstrates DNA-binding activity independently of SRF.
The purified ETS protein also binds SRF in vitro, indicating a dual interaction capability.

Conclusions:

The identified 60-kDa ETS protein is a novel factor that binds specific DNA sequences and interacts with SRF.
The SRF DNA-binding/oligomerization domain exhibits affinity for distinct ETS oncoproteins.
This finding suggests a complex regulatory network involving ETS proteins and SRF in gene expression.