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Nuclear localization of tumor necrosis factor-alpha in human osteoblast-like cells
1Department of Biochemistry and Molecular Biology, Graduate School of Biomedical Sciences, Newark, N.J.
Biochemical and Biophysical Research Communications
|June 30, 1994
Abstract:
We have studied the internalization and subsequent nuclear localization of [125I]-rTNF-alpha in a human osteoblast-like cell line, HOS TE85. TNF-alpha is rapidly internalized and optimum cellular levels are achieved in approximately 20 minutes at 37 degrees C. A portion of the internalized cytokine is translocated to the nucleus as judged by its presence in highly purified nuclei as a 17-kDa form.