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Purification of peptidyl-tRNA on benzoylated DEAE-cellulose columns
Nucleic Acids Research
|December 1, 1975
Abstract:
The o-nitrophenylsulfenyl group, and amino protecting group in the chemical synthesis of peptidyl-tRNA was used to attach newly synthesized peptidyl-tRNA to a benzoylated DEAE-cellulose column. This facilitated the isolation of a highly purified specific tRNA with a well defined peptide chain.