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Altered gut barrier function to Candida during parenteral nutrition
I Pappo1, I Polacheck, O Zmora
1Department of Surgery, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
Nutrition (Burbank, Los Angeles County, Calif.)
|March 1, 1994
Summary
Total parenteral nutrition (TPN) may increase the risk of Candida sepsis. This study suggests TPN-induced gut changes facilitate Candida translocation, leading to sepsis in rats.
Area of Science:
- Microbiology
- Gastroenterology
- Sepsis Research
Background:
- Catheter-related sepsis is a concern in patients receiving total parenteral nutrition (TPN).
- The gut microbiome's role in TPN-associated infections is not fully understood.
Purpose of the Study:
- To investigate the hypothesis that Candida translocation from the gut causes catheter-related sepsis during TPN.
- To explore the impact of TPN and bowel rest on gut barrier function in relation to Candida.
Main Methods:
- Male Sabra rats were randomized into four groups: controls, saline infusion, TPN infusion, and TPN with antibiotics.
- Groups received Candida albicans by gavage on day 7.
- Candida colony-forming units were quantified in blood, mesenteric lymph nodes, and kidneys 24 hours later.
Main Results:
- No Candida growth was observed in control or saline-infused groups.
- Candida was detected in blood, lymph nodes, and kidneys of rats receiving TPN (groups 3 and 4).
- Significant Candida levels were found in mesenteric lymph nodes of the TPN group.
Conclusions:
- Candida growth during TPN suggests altered gut barrier function.
- TPN and bowel rest may promote Candida translocation from the gut, potentially causing sepsis.
- Further research is needed to confirm the link between TPN, gut barrier dysfunction, and Candida sepsis.