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Related Experiment Videos

Reporter genes in transgenic mice

C Cui1, M A Wani, D Wight

  • 1Department of Anatomy and Cell Biology, University of Cincinnati, OH 45267-0521.

Transgenic Research
|May 1, 1994
PubMed
Summary

Reporter transgenes in transgenic animals offer powerful tools for studying gene expression and biological mechanisms. While challenges exist, particularly with postnatal in vivo applications, ongoing developments promise expanded research capabilities.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • Reporter transgenes, often of prokaryotic origin, encode detectable products not native to eukaryotic cells.
  • These transgenes are utilized in transgenic animals to investigate biological processes.
  • Reporter gene products can be detected via enzyme activity assays or in situ at the single-cell level.

Purpose of the Study:

  • To review the applications of reporter transgenes in transgenic animals.
  • To highlight the utility of reporter genes in dissecting biological questions.
  • To discuss limitations and future directions for reporter gene technology in vivo.

Main Methods:

  • Utilizing reporter transgenes in various animal models.
  • Employing reporter gene products for detection in cell/tissue homogenates or in situ.

Related Experiment Videos

  • Analyzing gene expression, development, cancer models, mutagenesis, lineage tracing, and cell marking.

    • Main Results:

    • Reporter genes have successfully identified regulatory elements and aided in creating in vivo models.
    • The lacZ reporter gene, while effective in early development, shows unreliable postnatal in vivo expression.
    • Transgenic mice expressing reporter genes provide valuable insights into in vivo biological mechanisms.

    Conclusions:

    • Reporter transgenes are invaluable tools for in vivo biological research in transgenic animals.
    • Limitations in current reporter gene systems, like lacZ's postnatal expression, necessitate further development.
    • Advancements in inducible/repressible transgene systems will enhance the capacity to address complex biological questions.