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Antiviral activity of mice with chronic renal failure--an assessment using peritoneal effluents
A Pomeranz1, O Smetana, J Ben-Dahan
1Department of Nephrology, Meir General Hospital, Kfar Saba, Israel.
Abstract:
Antiviral activity (AVA) determined by the inhibition of the cytopathic effect (CPE) of vesicular stomatitis virus (VSV) on mice fibroblasts, was measured in the peritoneal effluent of mice. Four groups of animals (each group numbering 30 mice) were studied. Group 1 consisted of sham operated mice and served as the control group. Group 2 underwent implantation of silastic matter (of which the Tenckhoff catheter is made). In group 3, chronic renal failure was induced. Group 4 comprised those mice in which both chronic renal failure was induced and silastic matter implanted. Optical density readings, directly related to the inhibition of CPE were 0.66 +/- 0.01, 0.59 +/- 0.08, 0.85 +/- 0.06 and 0.86 +/- 0.13 for groups 1, 2, 3 and 4, respectively (p < 0.01 for groups 1 and 2 versus 3 and 4). Virus control readings indicative of the CPE of VSV without the presence of peritoneal effluent were 0.58 +/- 0.07, not significantly different from those obtained in groups 1 and 2. They were, however, significantly below values from groups 3 and 4 (p < 0.05). These data show that AVA is undetectable in the peritoneal effluent of normal mice. Chronic renal failure produces an enhancement of AVA. Silastic matter (Tenckhoff catheter) implantation does not play a role in the production of AVA.
Insights
Antiviral activity is undetectable in normal mice but enhanced by chronic renal failure. Tenckhoff catheter implantation does not affect this antiviral activity in peritoneal effluent.
Area of Science:
- Nephrology
- Virology
- Immunology
Background:
- Antiviral activity (AVA) in peritoneal effluent is not well understood.
- Chronic renal failure (CRF) may alter immune responses.
- The impact of medical devices like Tenckhoff catheters on AVA is unknown.
Purpose of the Study:
- To investigate the presence and modulation of antiviral activity in mouse peritoneal effluent.
- To determine the effect of chronic renal failure on peritoneal antiviral activity.
- To assess the influence of silastic matter (Tenckhoff catheter material) on antiviral activity.
Main Methods:
- Four groups of mice were studied: control, silastic implant, CRF induction, and CRF with silastic implant.
- Antiviral activity was measured by the inhibition of cytopathic effect (CPE) of vesicular stomatitis virus (VSV) on mouse fibroblasts.
- Optical density readings were used to quantify CPE inhibition in peritoneal effluent.
Main Results:
- Antiviral activity was undetectable in the peritoneal effluent of normal mice (Groups 1 and 2).
- Significantly enhanced antiviral activity was observed in mice with chronic renal failure (Groups 3 and 4) compared to controls (p < 0.01).
- Silastic matter implantation (Tenckhoff catheter) did not significantly alter antiviral activity in either normal or CRF mice.
Conclusions:
- Peritoneal effluent from normal mice lacks detectable antiviral activity.
- Chronic renal failure significantly enhances antiviral activity in the peritoneal effluent.
- Tenckhoff catheter material does not contribute to the production of antiviral activity in peritoneal effluent.