Cloning and characterisation of the gene encoding cytochrome c4 from Pseudomonas stutzeri

By use of a degenerate oligodeoxyribonucleotide probe corresponding to the N-terminal amino acid (aa) sequence and Southern blot analysis, the gene encoding the di-heme cytochrome c4 (CC4) from Pseudomonas stutzeri has been cloned. The aa sequence deduced from the nucleotide sequence shows a 20-aa signal peptide and a 190-aa mature protein with 79% identity to the Azotobacter vinelandii CC4 sequence, which had earlier been determined by aa sequencing.