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Preliminary interpretive susceptibility testing criteria for FK-037 with 30-microgram disks
1Department of Pathology, University of Iowa College of Medicine, Iowa City 52242.
Abstract:
FK-037, a new parenteral cephalosporin, was tested against 483 organisms from clinical infections to establish preliminary susceptibility testing criteria with 30-micrograms disks. The proposed breakpoint zone diameters were > or = 17 mm (MIC correlate, < or = 8 micrograms/ml) for the susceptible category and < or = 13 mm (MIC correlate, > or = 32 micrograms/ml) for the resistant category. These interpretive guidelines produced no very major (false-susceptible) errors, 2.9% major (false-resistant) errors, and 6.2% minor errors for a total absolute agreement between methods of 90.9%.
Insights
FK-037, a new parenteral cephalosporin antibiotic, demonstrated effective antimicrobial susceptibility testing criteria. Proposed guidelines for FK-037 disk diffusion testing showed high accuracy in classifying bacterial susceptibility and resistance.
Area of Science:
- Clinical Microbiology
- Infectious Diseases
- Pharmacology
Background:
- Parenteral cephalosporins are crucial in treating bacterial infections.
- Establishing reliable susceptibility testing criteria is vital for effective antibiotic therapy.
- FK-037 is a novel parenteral cephalosporin requiring validated testing methods.
Purpose of the Study:
- To determine preliminary susceptibility testing criteria for FK-037.
- To evaluate the accuracy of proposed breakpoint zone diameters using 30-micrograms disks.
- To assess the performance of FK-037 interpretive guidelines against clinical isolates.
Main Methods:
- Tested FK-037 against 483 bacterial organisms from clinical infections.
- Utilized 30-micrograms disks for susceptibility testing.
- Determined zone diameters and correlated them with Minimum Inhibitory Concentration (MIC) values.
Main Results:
- Proposed breakpoints: >= 17 mm (susceptible, MIC <= 8 µg/ml) and <= 13 mm (resistant, MIC >= 32 µg/ml).
- Achieved 90.9% absolute agreement between disk diffusion and MIC methods.
- Reported 0% very major errors, 2.9% major errors, and 6.2% minor errors.
Conclusions:
- The proposed susceptibility testing criteria for FK-037 are accurate and reliable.
- These guidelines minimize errors in classifying bacterial susceptibility to FK-037.
- Validated criteria will aid in the appropriate clinical use of FK-037.