Related Experiment Videos
Chemiluminescent hybridization assay for hepatitis delta virus RNA in serum
M Gallagher1, T T Morris, H A Fields
1Hepatitis Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
Journal of Clinical Microbiology
|April 1, 1994
Abstract:
A nonradioactive hybridization assay for the detection of hepatitis delta virus RNA in serum is described. This assay utilizes a digoxigenin-labeled RNA hybridization probe and chemiluminescent immunodetection. The probe can detect as little as 0.4 pg of cDNA with a 60-min exposure. Results obtained are in agreement with serological indicators of hepatitis delta virus infection and are comparable to those obtained by hybridization assays employing radioactively labeled RNA.