Related Experiment Video
Updated: Aug 3, 2026

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation
Published on: July 4, 2015
DNA repair after UV and gamma irradiation. I. Rat spleen cells
Abstract:
An in vitro microculture was set up to standardize DNA enzymatic repair in rat spleen lymphocytes after exposure to ultraviolet or gamma rays by measuring the tritiated thymidine uptake. A relation between irradiation dose and DNA repair is always observable when cell viability and scheduled DNA synthesis are taken into account. This micromethod utilizing rat spleen cells appears to be a suitable system for such a study since it permits a satisfactory evaluation of DNA repair.
Related Concept Videos
Nucleotide Excision Repair
Overview of DNA Repair
Chemically...
Nucleotide Excision Repair
Mutations
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
Nucleotide Excision Repair
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Other Unique Bacteria

