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Published on: September 7, 2017
DNA modification promoted by water-soluble nickel(II) salen complexes: a switch to DNA alkylation
J G Muller1, S J Paikoff, S E Rokita
1Department of Chemistry, State University of New York at Stony Brook 11794-3400.
Abstract:
Reaction of a 17-base hairpin-forming oligonucleotide with [N,N'-bis(salicylaldehyde)-meso-1,2-bis(4- trimethylaminophenyl)ethylenediimino]nickel(II) perchlorate, 2, and KHSO5 produced two types of high molecular weight products, an alkaline-labile species and a nonalkaline-labile species, which co-migrated on gel electrophoresis. Upon treatment with piperidine, the base-labile derivative led to strand scission products only at accessible guanine residues that were not part of a Watson-Crick duplex. The formation of higher molecular weight species is proposed to occur via a highly reactive ligand-centered radical acting as a DNA alkylating agent.
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