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Crystallization and preliminary X-ray diffraction analysis of a type I beta-glucosidase encoded by the bgIA gene of
J Sanz-Aparicio1, A Romero, M Martinez-Ripoll
1Departamento de Cristalografía, Instituto de Química-Física Rocasolano C.S.I.C., Madrid, Spain.
Journal of Molecular Biology
|July 15, 1994
Abstract:
The enzyme encoded by the bgIA gene of Bacillus polymyxa, a type I beta-glucosidase belonging to family I of glycosyl hydrolases, has been purified to homogeneity from an Escherichia coli culture which overexpressed the gene, and crystallized. The crystals, which diffract to 3.0 A resolution, belong to the orthorhombic space group C222(1). The cell dimensions are a = 155.4 A, b = 209.4 A, c = 209.7 A.