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Identification of GABAA receptor subunits expressed in bovine adrenal medulla
M Parramón1, M J Oset-Gasque, M P Gonzalez
1Instituto de Bioquímica, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Abstract:
The GABAA receptor subunits expressed in adrenal medulla have been characterized by radioligand binding and by immunological methods. The receptors were purified by both benzodiazepine affinity chromatography and anti-alpha 1 413-429 GABAA receptor antibody immunoaffinity chromatography. These preparations were screened by immunoblotting using GABA receptor alpha subunit sequence-specific antibodies. Results showed the existence of the alpha 1 subunit. Thus, the GABAA receptors expressed in adrenal medulla are homogeneous with respect to their alpha subunit complement and consistent with type BZ1 benzodiazepine receptor pharmacology.