Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microtubules stability in human peripheral nerves

N Oka1, T Nishio, I Akiguchi

  • 1Department of Neurology, Faculty of Medicine, Kyoto University, Japan.

Neuroscience Letters
|February 28, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HtrA2/Omi-immunoreactive intraneuronal inclusions in the anterior horn of patients with sporadic and Cu/Zn superoxide dismutase (SOD1) mutant amyotrophic lateral sclerosis.

Neuropathology and applied neurobiology·2010
Same author

Cytopathological alterations and therapeutic approaches in Binswanger's disease.

Neuropathology : official journal of the Japanese Society of Neuropathology·2009
Same author

Anti-DARPP32 antibody-immunopositive inclusions in the brain of patients with multiple system atrophy.

Clinical neuropathology·2008
Same author

Age-related changes in barrier function in mouse brain II. Accumulation of serum albumin in the olfactory bulb of SAM mice increased with aging.

Archives of gerontology and geriatrics·2008
Same author

The relationship between 24-hour blood pressure readings, subcortical ischemic lesions and vascular dementia.

Cerebrovascular diseases (Basel, Switzerland)·2005
Same author

CD16+CD57- natural killer cells in multifocal motor neuropathy.

European neurology·2005

Microtubules (MT) in nerves show cold instability, but a cold-stable tubulin subpopulation exists. Acute dysautonomia patients exhibit reduced cold-stable MT and tubulin, indicating altered axonal degeneration processes.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Microtubules (MT) are crucial cytoskeletal components in axons.
  • Their stability is vital for neuronal structure and function.
  • Cold-induced destabilization of MT is a known phenomenon.

Purpose of the Study:

  • To investigate the cold stability of microtubules in human sural nerve axons.
  • To compare cold stability in control subjects versus patients with acute dysautonomia.
  • To explore the potential existence of cold-stable tubulin subpopulations.

Main Methods:

  • Morphological analysis of microtubule density in cold-treated sural nerve biopsies.
  • Biochemical extraction studies to quantify cold-insoluble tubulin.
  • Comparative analysis between control nerves and nerves from patients with acute dysautonomia.

Related Experiment Videos

Main Results:

  • Cold treatment reduced microtubule density in control unmyelinated axons.
  • Morphological studies showed ~30% cold-insoluble MT, while extraction revealed 60-75% cold-insoluble tubulin.
  • Patients with acute dysautonomia displayed decreased cold-stable MT and tubulin compared to controls.

Conclusions:

  • A subpopulation of cold-stable tubulin may exist independently of assembled microtubules.
  • Acute dysautonomia is associated with reduced cold stability of microtubules and tubulin.
  • These findings suggest altered microtubule dynamics in primary axonal degeneration associated with acute dysautonomia.