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Evolutionary conservation of a human function-associated molecule on murine natural killer cells: expression and
R Kapur1, D L Evans, D T Harris
1Department of Microbiology and Immunology, University of Arizona, Tucson 85721.
Scandinavian Journal of Immunology
|July 1, 1994
Summary
A novel function-associated molecule (FAM) on natural killer (NK) cells is identified. Anti-FAM monoclonal antibodies (MoAbs) inhibit NK cell cytotoxicity and recognition, highlighting FAM
Area of Science:
- Immunology
- Cell Biology
Background:
- Natural killer (NK) cells are crucial for innate immunity.
- A conserved function-associated molecule (FAM) has been identified on NK cells across species.
Purpose of the Study:
- To investigate the presence and function of FAM on murine NK cells.
- To characterize FAM expression in different NK cell populations and tissues.
Main Methods:
- Two-colour flow cytometry using anti-FAM monoclonal antibody (MoAb) 5C6.
- Analysis of NK cell subpopulations, including lymphokine-activated killer (LAK) and adherent LAK (ALAK) cells.
- Cytotoxicity and conjugate formation assays.
Main Results:
- FAM is expressed on a subpopulation of murine NK cells (NK1.1+).
- FAM expression is higher on activated NK cells (LAK, ALAK) and in splenocytes.
- Anti-FAM MoAb 5C6 inhibits NK cell cytotoxicity and target cell recognition.
Conclusions:
- FAM is a conserved molecule involved in murine NK cell function.
- FAM plays a role in NK cell-mediated cytotoxicity and cell-cell recognition.