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Related Experiment Videos

Biochemical changes of ischemia

S Levitsky1, H Feinberg

  • 1Department of Surgery, Abraham Lincoln School of Medicine, University of Illinois College of Medicine, Chicago 60680.

The Annals of Thoracic Surgery
|July 1, 1975
PubMed
Summary

Normothermic ischemic arrest significantly alters myocardial metabolism, reducing high-energy phosphates and glycogen. This metabolic shift impairs cardiac contractility recovery even after reperfusion.

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Area of Science:

  • Cardiology
  • Biochemistry
  • Physiology

Background:

  • Normothermic ischemic arrest via aortic cross-clamping is a common surgical procedure.
  • The metabolic consequences of this procedure on the myocardium are not fully understood.

Purpose of the Study:

  • To investigate the effects of aortic cross-clamping on myocardial glycolytic pathways.
  • To assess associated morphological changes and high-energy phosphate conservation.

Main Methods:

  • Analysis of myocardial metabolic changes during and after normothermic ischemic arrest.
  • Measurement of high-energy phosphates, glycogen, and lactate production.

Main Results:

  • A significant reduction in total high-energy phosphates (62%) and glycogen (63%) was observed.
  • Lactate production increased by 243%, indicating a shift to anaerobic metabolism.
  • High-energy nucleotides remained depressed even after reperfusion.

Conclusions:

  • Normothermic ischemic arrest induces significant alterations in myocardial carbohydrate metabolism.
  • Persistent metabolic abnormalities and low high-energy nucleotide levels hinder the recovery of cardiac contractility post-arrest and reperfusion.

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