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Cross-reactivity of ovine IgE with anti-human IgE
H Yilmaz1, K L Morgan, J M Roe
1School of Veterinary Science, Department of Veterinary Medicine, Bristol, UK.
International Archives of Allergy and Immunology
|July 1, 1994
Abstract:
Cross-reactivity between ovine and human IgE was investigated using monoclonal antibodies to human IgE heavy chain. Western immunoblotting, reverse cutaneous anaphylaxis (RCA), passive cutaneous anaphylaxis inhibition (PCAI) and reverse passive cutaneous anaphylaxis (RPCA) tests were all used to assess cross-reactivity between the monoclonals and ovine IgE. No cross-reactivity was demonstrated using Western immunoblotting, RCA and PCAI tests. However, evidence for homology in the region of the molecule concerned with mast cell binding was demonstrated by RPCA performed in sheep skin primed with human myeloma IgE.