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Internalization of surface components during Fc-receptor mediated phagocytosis by macrophages

D de F Buchi1, W de Souza

  • 1Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.

Mouse peritoneal resident macrophages efficiently ingest IgG-coated erythrocytes through a phagocytic process mediated by Fc-receptors. During this process surface components of the macrophages, which can be labeled by cationized ferritin and gold-labeled lectins are not interiorized together with the IgG-coated erythrocytes. However, they are internalized through endocytic vesicles and concentrated into vacuoles which are part of the endosomal-lysosomal system. Later on some of these components either are recycled back to the cell surface or are released, due to a process of lysosome-phagosome fusion, into the erythrocyte-containing phagosomes.

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