Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sulfation pharmacogenetics in humans

R Weinshilboum1, I Aksoy

  • 1Department of Pharmacology, Mayo Medical School, Rochester, MN 55905.

Chemico-Biological Interactions
|June 1, 1994
PubMed
Summary

Genetic variations influence sulfotransferase (ST) enzyme activity in human platelets and liver. Platelet ST activity correlates with other tissues, suggesting shared genetic regulation, while liver DHEA ST shows potential genetic polymorphism.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Low-dose digoxin in patients with heart failure with reduced or mildly reduced ejection fraction: a randomized controlled trial.

Nature medicine·2026
Same author

Evaluation of YouTube Videos on Defibrillation Applications in Cardiopulmonary Resuscitation: A Comprehensive Analysis.

Nigerian journal of clinical practice·2024
Same author

The impact of patient-reported frailty on cardiovascular outcomes in elderly patients after non-ST-acute coronary syndrome.

International journal of cardiology·2024
Same author

The International SSRI Pharmacogenomics Consortium (ISPC): a genome-wide association study of antidepressant treatment response.

Translational psychiatry·2016
Same author

Polymorphic variation in TPMT is the principal determinant of TPMT phenotype: A meta-analysis of three genome-wide association studies.

Clinical pharmacology and therapeutics·2016
Same author

[Fatal aortarupture following electroconvulsive therapy].

Tijdschrift voor psychiatrie·2016

Area of Science:

  • Biochemistry
  • Pharmacogenetics
  • Human Physiology

Background:

  • Cytoplasmic sulfotransferase (ST) enzymes, including thermostable (TS) and thermolabile (TL) forms of PST, and dehydroepiandrosterone (DHEA) ST, are present in human tissues.
  • Blood platelets express both TS and TL PST, enabling pharmacogenetic studies of these enzymes in humans.

Purpose of the Study:

  • To investigate the genetic regulation of TS and TL PST activity in human platelets.
  • To explore the potential genetic regulation of DHEA ST activity in human liver tissue.

Main Methods:

  • Pharmacogenetic analysis of ST enzyme activity in human blood platelets.
  • Biochemical assays of DHEA ST activity in human hepatic biopsy samples.
  • Analysis of DHEA ST activity distribution for evidence of genetic polymorphism.

Main Results:

  • TS and TL PST activities in platelets are regulated by distinct genetic polymorphisms.
  • Platelet TS PST activity correlates with enzyme levels in liver, jejunum, and cerebral cortex.
  • Human hepatic DHEA ST activity exhibits a 4.6-fold range and a bimodal distribution, suggesting genetic regulation.

Conclusions:

  • Genetic polymorphisms play a significant role in regulating human platelet ST enzyme activity.
  • DHEA ST activity in the liver may also be influenced by genetic factors, warranting further molecular investigation.
  • Understanding the genetic basis of ST enzyme variation is crucial for determining the role of inheritance in drug, xenobiotic, neurotransmitter, and hormone metabolism.

Related Experiment Videos