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Androgen and estrogen sulfotransferases of the rat liver: physiological function, molecular cloning, and in vitro
B Chatterjee1, C S Song, J M Kim
1Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio 78284.
Chemico-Biological Interactions
|June 1, 1994
Abstract:
Cloning of the androgen and estrogen sulfotransferases has allowed studies on their spatiotemporal regulation and physiological function. These two enzymes appear to be involved in regulating target cell sensitivity for the androgenic and estrogenic steroids. Recombinant androgen sulfotransferase produced in insect Sf9 cells through a baculoviral expression vector is capable of transferring the sulfate from PAPS (phosphoadenosine phosphosulfate) to 3 alpha, 3 beta, 17 alpha, and 17 beta hydroxyl groups of a number of steroid substrates indicating a broad range of substrate specificity.