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A preserved leukocyte preparation for quality control

D Pinthanond, B Petchclai

    American Journal of Clinical Pathology
    |January 1, 1975
    PubMed
    Summary

    This study presents a new method for stabilizing leukocytes (white blood cells) for quality control. These stabilized leukocyte preparations remain stable for at least three months at various temperatures.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Laboratory Medicine

    Background:

    • Accurate quality control of leukocyte preparations is crucial for reliable diagnostic and research applications.
    • Existing methods for leukocyte preservation may have limitations in stability and duration.

    Purpose of the Study:

    • To develop and describe a novel preparation technique for stabilized human leukocytes.
    • To assess the stability and viability of these leukocyte preparations over time and at different storage conditions.

    Main Methods:

    • Human leukocytes were isolated from blood using dextran and erythrocytes were lysed.
    • Leukocytes were washed, fixed with glutaraldehyde, and resuspended in a saline solution with sodium azide.
    • Preparations were stored at 4°C and room temperature for stability assessment.

    Main Results:

    • The described preparation technique yielded highly stable leukocyte samples.
    • Stability was maintained for at least 3 months at both tested storage temperatures (4°C and room temperature).
    • Stability was confirmed using both manual (May-Grünwald staining) and automated electronic cell counting methods.

    Conclusions:

    • The developed method provides a robust and stable preparation of human leukocytes suitable for quality control purposes.
    • This technique offers a reliable solution for preserving leukocyte integrity for extended periods, enhancing laboratory efficiency and data accuracy.

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