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Contact sensitivity to Tinuvin P in mice
Y Ikarashi1, T Tsuchiya, A Nakamura
1Division of Medical Devices, National Institute of Hygienic Sciences, Tokyo, Japan.
Contact Dermatitis
|April 1, 1994
Summary
2-(2'-hydroxy-5'-methylphenyl)benzotriazole (Tinuvin P) can cause skin sensitization, as shown by modified mouse ear swelling tests. However, Tinuvin P-sensitized mice did not cross-react with other benzotriazole UV absorbers, indicating specific sensitization.
Area of Science:
- Dermatology
- Toxicology
- Immunology
Background:
- Benzotriazole UV absorbers are widely used in various materials.
- Assessing the skin sensitization potential of chemical compounds is crucial for safety evaluations.
- 2-(2 ahydroxy-5 ahydroxymethylphenyl)benzotriazole (Tinuvin P) is a common UV absorber.
Purpose of the Study:
- To investigate the contact sensitizing potential of Tinuvin P.
- To evaluate cross-reactivity between Tinuvin P and other benzotriazole UV absorbers.
Main Methods:
- Murine Local Lymph Node Assay (LLNA)
- Modified Mouse Ear Swelling Test (MEST) with modified induction procedure
- Cross-reaction tests with other benzotriazole UV absorbers
Main Results:
- Topical exposure to Tinuvin P did not induce significant changes in lymph node weight or cell proliferation in LLNA.
- Tinuvin P showed negative results in the standard LLNA, indicating weak sensitizing potential.
- Modified MEST demonstrated that Tinuvin P has contact sensitizing ability.
- No cross-reactivity was observed between Tinuvin P-sensitized mice and other tested benzotriazole UV absorbers.
Conclusions:
- Tinuvin P possesses contact sensitizing properties.
- Sensitization to Tinuvin P does not confer cross-reactivity to other structurally similar benzotriazole UV absorbers.