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Updated: Aug 14, 2026

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
Published on: September 16, 2014
An increase of the energy coupling capacity of submitochondrial particles by lanthanides
V G Grivennikova1, E V Gavrikova, A D Vinogradov
1Department of Biochemistry, School of Biology, Moscow State University, Russian Federation.
Abstract:
NADH and succinate oxidase activities of inside-out submitochondrial particles treated with excess oligomycin are inhibited by lanthanides (La3+ and Dy3+). Both inhibition by oligomycin and oligomycin plus lanthanides are completely relieved by an uncoupler. The respiratory control measured as the stimulation of NADH or succinate oxidation caused by the addition of uncoupler to the oligomycin-treated particles is thus increased in the presence of lanthanides. The coupling effect of lanthanides is completely prevented and rapidly reversed by excess EDTA. La3+ increases the extent of the aerobic energy-linked succinate-supported NAD+ reduction catalyzed by the oligomycin-treated submitochondrial particles. Lanthanides seem to be useful tools to increase the energy coupling capacity of the submitochondrial particles.
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