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Related Experiment Videos

Listeria cell wall fraction: a B cell mitogen

J J Cohen, G E Rodriguez, P D Kind

    Journal of Immunology (Baltimore, Md. : 1950)
    |March 1, 1975
    PubMed
    Summary

    Listeria cell wall fraction (LCWF) acts as a B cell mitogen in mice, stimulating immune responses. This substance differs from endotoxin and PPD, suggesting a unique role in Listeria resistance.

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    Area of Science:

    • Immunology
    • Microbiology
    • Cell Biology

    Background:

    • Listeria monocytogenes infection triggers immune responses.
    • Understanding the immunomodulatory components of Listeria is crucial for developing effective treatments.

    Purpose of the Study:

    • To identify and characterize substances within Listeria monocytogenes cell walls that stimulate immune cells.
    • To determine the mitogenic activity and cellular targets of Listeria cell wall fraction (LCWF).

    Main Methods:

    • Preparation of a crude cell wall-rich fraction from Listeria monocytogenes.
    • Assessing blast transformation and thymidine incorporation in mouse spleen cells.
    • Testing mitogenic activity in various species, including mice (C3H/HeJ), rats, and humans.

    Main Results:

    • Listeria cell wall fraction (LCWF) induced blast transformation and thymidine incorporation in mouse spleen cells.
    • LCWF demonstrated B cell mitogenic activity in mice, including C3H/HeJ mice, indicating it is not endotoxin.
    • LCWF showed weak mitogenicity in rat spleen cells and no mitogenicity in human lymphocytes.

    Conclusions:

    • Listeria cell wall fraction (LCWF) is a potent B cell mitogen in mice with a distinct activity spectrum.
    • LCWF's properties differentiate it from endotoxin and purified protein derivative (PPD).
    • A cell wall-associated mitogen may contribute to early resistance against Listeria infections.

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