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Colonization and antibody response in mice and rats experimentally infected with Pasteurellaceae from different
R Boot1, H C Thuis, J L Veenema
1Section of Laboratory Animal Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Abstract:
Mice and rats were experimentally infected with Pasteurellaceae isolated from mice, rats, hamsters and gerbils. Mice and rats were most heavily colonized by strains originally isolated from mice and rats respectively, and to a lesser extent by Pasteurellaceae from hamsters and gerbils. Colonization was generally accompanied by seroconversion. Gross pathology of the lungs was not observed. We conclude that Pasteurellaceae-free SPF mice and rats can be colonized by members of this bacterial family present in other rodent species.
Insights
SPF mice and rats can be colonized by Pasteurellaceae from other rodents. This bacterial family colonization, observed in laboratory rodents, did not cause lung pathology but did induce seroconversion.
Area of Science:
- Microbiology
- Veterinary Science
- Rodent Health
Background:
- Pasteurellaceae are common bacteria found in rodents.
- Understanding interspecies transmission of Pasteurellaceae is crucial for animal models.
- SPF rodents are typically free from Pasteurellaceae.
Purpose of the Study:
- To investigate the colonization potential of Pasteurellaceae from various rodent species in SPF mice and rats.
- To determine if Pasteurellaceae colonization leads to observable pathology.
- To assess the immune response (seroconversion) following colonization.
Main Methods:
- Experimental infection of SPF mice and rats with Pasteurellaceae isolates from mice, rats, hamsters, and gerbils.
- Assessment of colonization levels in different rodent hosts.
- Gross pathological examination of lungs.
- Serological testing to detect antibody response.
Main Results:
- Mice and rats showed highest colonization by Pasteurellaceae strains originally isolated from mice and rats, respectively.
- Lower levels of colonization were observed with Pasteurellaceae from hamsters and gerbils.
- Colonization was generally associated with seroconversion.
- No gross lung pathology was detected in any experimental group.
Conclusions:
- Pasteurellaceae-free SPF mice and rats are susceptible to colonization by Pasteurellaceae from other rodent species.
- Interspecies transmission of Pasteurellaceae among rodents is feasible.
- Colonization by these bacteria does not necessarily result in overt lung disease but elicits an immune response.