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RNA subunit structure of Mason-Pfizer monkey virus

G Schochetman, J Schlom

    Journal of Virology
    |February 1, 1975
    PubMed
    Summary

    The Mason-Pfizer monkey virus 60-70S RNA, with a molecular weight of 8x10^6 daltons, dissociates into identical 2.8x10^6 dalton subunits. Partial dissociation reveals three distinct low-molecular-weight RNA species.

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    Area of Science:

    • Virology
    • Molecular Biology
    • Biochemistry

    Background:

    • The Mason-Pfizer monkey virus (MPMV) is a retrovirus known to possess a large 60-70S RNA genome.
    • Understanding the subunit structure of viral RNA is crucial for comprehending viral replication and assembly.

    Purpose of the Study:

    • To investigate the molecular weight and dissociation characteristics of Mason-Pfizer monkey virus 60-70S RNA.
    • To identify the subunit structures and any smaller RNA species released upon dissociation.

    Main Methods:

    • Analysis of Mason-Pfizer monkey virus 60-70S RNA using polyacrylamide gel electrophoresis.
    • Dissociation of the 60-70S RNA using heat and varying concentrations of formamide.

    Main Results:

    • The native Mason-Pfizer monkey virus 60-70S RNA exhibits a molecular weight of 8x10^6 daltons.
    • Complete dissociation by heat or 40% formamide yields identical subunit structures of 2.8x10^6 daltons.
    • Partial dissociation with lower formamide concentrations releases three low-molecular-weight RNA species: 10^5, 3.5x10^4, and 2.5x10^4 daltons.

    Conclusions:

    • Mason-Pfizer monkey virus 60-70S RNA is composed of identical subunits.
    • The dissociation pattern suggests a complex organization of the viral genome, with potential for smaller functional RNA molecules.

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