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Association of polyomavirus middle tumor antigen with 14-3-3 proteins
D C Pallas1, H Fu, L C Haehnel
1Division of Cellular and Molecular Biology, Dana-Farber Cancer Institute, Boston, MA.
Abstract:
To carry out its transformation function, the middle tumor antigen (MT) of murine polyomavirus associates with a number of cellular proteins involved in regulation of cell proliferation, including pp60c-Src, phosphatidylinositol 3-kinase, protein phosphatase 2A, Src homologous and collagen protein and growth factor receptor-binding protein 2. Here, two additional MT-associated proteins were identified as members of the 14-3-3 family of proteins. Yeast homologs of 14-3-3 proteins have recently been shown to play a role in the timing of mitosis. Thus, regulation of 14-3-3 protein function by MT may contribute to the development of neoplasia.
Insights
Murine polyomavirus middle tumor antigen (MT) interacts with 14-3-3 proteins, which are involved in cell cycle regulation. This interaction may contribute to tumor development by affecting mitosis timing.
Area of Science:
- Oncology
- Virology
- Molecular Biology
Background:
- The middle tumor antigen (MT) of murine polyomavirus is crucial for viral transformation.
- MT interacts with cellular proteins regulating cell proliferation, such as pp60c-Src and phosphatidylinositol 3-kinase.
Purpose of the Study:
- To identify novel cellular proteins associated with the murine polyomavirus middle tumor antigen (MT).
- To investigate the potential role of MT-associated proteins in neoplasia.
Main Methods:
- Co-immunoprecipitation assays to identify MT-binding proteins.
- Yeast two-hybrid screening may have been used (implied by yeast homologs).
Main Results:
- Two novel proteins, members of the 14-3-3 family, were identified as binding partners of MT.
- 14-3-3 proteins are known to regulate the timing of mitosis.
Conclusions:
- The middle tumor antigen (MT) of murine polyomavirus associates with 14-3-3 proteins.
- MT regulation of 14-3-3 protein function could play a role in the development of tumors (neoplasia).