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The primary structure of rat ribosomal protein S23
Y Kitaoka1, J Olvera, I G Wool
1Department of Biochemistry and Molecular Biology, University of Chicago, Illinois 60637.
Biochemical and Biophysical Research Communications
|July 15, 1994
Summary
Researchers identified the rat ribosomal protein S23 sequence, finding it has 142 amino acids and is conserved across species. This protein
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Ribosomal proteins are essential components of ribosomes, the cellular machinery responsible for protein synthesis.
- Understanding the structure and genetics of ribosomal proteins is crucial for comprehending gene expression and cellular function.
Purpose of the Study:
- To determine the amino acid sequence of the rat 40S ribosomal subunit protein S23.
- To investigate the gene copy number and mRNA characteristics of rat ribosomal protein S23.
- To explore the evolutionary conservation of rat ribosomal protein S23.
Main Methods:
- Deduction of amino acid sequence from recombinant cDNA nucleotide sequence.
- Analysis of molecular weight and post-translational modifications.
- Hybridization of cDNA to nuclear DNA digests to estimate gene copy number.
- Determination of mRNA length.
Main Results:
- The amino acid sequence of rat ribosomal protein S23 was elucidated, revealing a protein of 142 amino acids with a molecular weight of 15,666.
- The N-terminal methionine is removed post-translationally.
- Gene copy number for S23 in rat nuclear DNA ranges from 6 to 13 copies.
- The mRNA for S23 is approximately 650 nucleotides long.
- Rat S23 shows high identity to human ribosomal protein and evolutionary relatedness to yeast and prokaryotic ribosomal proteins.
Conclusions:
- Rat ribosomal protein S23 is a highly conserved protein, indicating its fundamental role in ribosome function across diverse organisms.
- The genetic organization suggests a regulated expression of the S23 gene.
- This study provides a foundation for further research into the structure-function relationships of ribosomal proteins.