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Polarographic assay of hydrogen peroxide accumulation in microbial cultures
Applied Microbiology
|February 1, 1975
Abstract:
A method is described for determining low concentrations of hydrogen peroxide by using a polarographic oxygen electrode to measure the oxygen released into solution on addition of catalase. A sample can be assayed directly without prior manipulation in 3 min. The method is capable of assaying hydrogen peroxide concentrations as low as 7 muM. The method has proved extremely useful for the assay of hydrogen peroxide secreted into milk by lactic acid bacteria.