Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Keratan sulphate--a 'reserve' polysaccharide?

J E Scott1

  • 1Manchester University, U.K.

European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies
|April 1, 1994
PubMed
Summary

Keratan sulphate, a key component in avascular tissues, may substitute for chondroitin sulphate under low oxygen conditions. This polysaccharide likely functions as a reserve, maintaining tissue structure and organization when oxygen is scarce.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Medium-term dexa analysis of an uncemented femoral component.

Hip international : the journal of clinical and experimental research on hip pathology and therapy·2017
Same author

Postoperative cognitive dysfunction and its relationship to cognitive reserve in elderly total joint replacement patients.

Journal of clinical and experimental neuropsychology·2016
Same author

Depression and anxiety after total joint replacement among older adults: a meta-analysis.

Aging & mental health·2015
Same author

The ontogeny of the chin: an analysis of allometric and biomechanical scaling.

Journal of anatomy·2015
Same author

Incidence of delirium following total joint replacement in older adults: a meta-analysis.

General hospital psychiatry·2015
Same author

Passive agglutination method using collagen-coated tanned sheep erythrocytes to demonstrate collagen-glycosaminoglycan interaction.

Annals of the rheumatic diseases·2014

Area of Science:

  • Biochemistry
  • Connective Tissue Biology
  • Glycosaminoglycans

Background:

  • Early research on keratan sulphate and chondroitin sulphate structures obscured their relationship.
  • The fundamental polymer backbone of both is poly(Gal beta 1:4 Glc beta 1-3).
  • Helmut Greiling's group clarified keratan sulphate's complex sulphation patterns using a domain structure concept.

Purpose of the Study:

  • To describe the early history of keratan sulphate and its proteoglycans.
  • To explore the hypothesis that keratan sulphate acts as a functional substitute for chondroitin sulphate in oxygen-deprived environments.
  • To explain the distribution and ratios of keratan sulphate and chondroitin sulphate in various tissues based on oxygen availability.

Main Methods:

  • Historical review of keratan sulphate research.
  • Analysis of sulphation patterns and domain structures.
  • Correlation of keratan sulphate distribution with tissue oxygenation levels.

Main Results:

  • Keratan sulphate is prevalent in avascular tissues like cartilage, intervertebral discs, and corneal stroma.
  • Stockwell's hypothesis suggests keratan sulphate compensates for poor oxygen supply.
  • Keratan:chondroitin sulphate ratios in discs and corneas correlate with age and species, supporting the oxygen-deficiency hypothesis.

Conclusions:

  • Keratan sulphate may serve as a 'reserve' polysaccharide, performing chondroitin sulphate's role during oxygen scarcity.
  • The NAD:NADH ratio is a probable biochemical control point.
  • Both keratan and chondroitin/dermatan sulphates contribute to swelling the collagenous matrix and organizing connective tissues.

Related Experiment Videos