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Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
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Published on: May 22, 2012

Overview of international PCR standardization efforts

L E Bockstahler1

  • 1Molecular Biology Branch, Food and Drug Administration, Rockville, Maryland 20857.

PCR Methods and Applications
|April 1, 1994
PubMed
Abstract

No abstract available in PubMed .

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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