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Related Experiment Videos

Proficiency testing in reticulocyte counting

R C Lohmann1, L N Crawford, D E Wood

  • 1Hematology Laboratory, Victoria Hospital Corporation, London, Ontario, Canada.

Clinical and Laboratory Haematology
|March 1, 1994
PubMed
Summary
This summary is machine-generated.

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Manual reticulocyte counting, a key clinical laboratory test, shows significant inaccuracy. Automated methods are recommended for improved patient care quality.

Area of Science:

  • Clinical laboratory science
  • Hematology

Background:

  • The Laboratory Proficiency Testing Program in Ontario has evaluated clinical laboratories since 1974.
  • Reticulocyte count testing was incorporated in 1982 to ensure quality patient care.

Purpose of the Study:

  • To assess the proficiency of clinical laboratories in performing manual reticulocyte counts.
  • To evaluate the accuracy and precision of indirect counting methods for reticulocytes.

Main Methods:

  • Distribution of stained blood films to participating laboratories.
  • Performance assessment based on accuracy and precision compared to an all-methods' mean.
  • Reliability evaluation using the coefficient of variation (CV) within the cohort.

Main Results:

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  • Manual reticulocyte counting exhibited a common CV ranging from 25 to 30%.
  • Accuracy and precision were not significantly influenced by the manual counting system or laboratory type.
  • The inherent technological limitations of manual counting contribute to observed inaccuracies.

Conclusions:

  • Manual reticulocyte counting is technologically obsolete due to inherent inaccuracies.
  • While potentially useful qualitatively, automated methods are recommended for superior performance.
  • Transitioning to automated reticulocyte counting is advised for enhanced clinical laboratory practice.