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Related Experiment Videos

Leukocyte-extracellular matrix interactions in psoriasis

A I el-Sherif1, A Krupinski, S Majewski

  • 1Department of Dermatology, Warsaw School of Medicine, Poland.

International Journal of Dermatology
|May 1, 1994
PubMed
Summary

Psoriasis involves leukocyte migration influenced by cell-extracellular matrix (ECM) interactions. Reduced peripheral blood mononuclear cell (PBMC) adherence to ECM components in psoriasis may be treatable with etretinate.

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Area of Science:

  • Dermatology
  • Immunology
  • Cell Biology

Background:

  • Psoriasis pathogenesis involves directed leukocyte migration.
  • Leukocyte migration is significantly influenced by cell-extracellular matrix (ECM) interactions.

Purpose of the Study:

  • To investigate leukocyte-ECM interactions in psoriasis.
  • To evaluate the adherence of peripheral blood mononuclear cells (PBMC) and polymorphonuclear leukocytes (PMNL) to ECM components.

Main Methods:

  • Investigated adherence of PBMC and PMNL to collagen type I, IV, laminin, and fibronectin.
  • Utilized crystal violet staining and absorbance measurements at 570 nm.

Main Results:

  • Decreased PBMC adherence to collagen type IV in early psoriasis.

Related Experiment Videos

  • Reduced adherence to all ECM components, particularly collagen type IV and laminin, in extensive plaque psoriasis.
  • Increased PMNL adherence to collagen type IV in short-duration psoriasis.
  • Preliminary evidence suggests etretinate may modulate leukocyte adherence to ECM.

    • Conclusions:

    • Decreased in vitro PBMC adherence to ECM, especially basement membrane components, suggests in vivo activation and migration to the epidermis in psoriasis.
    • This leukocyte-ECM interaction is a fundamental aspect of psoriasis.
    • Etretinate therapy may influence these leukocyte-ECM interactions in psoriasis patients.