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Related Experiment Videos

vanA-mediated vancomycin-resistant Enterococcus spp. in sewage

C Torres1, J A Reguera, M J Sanmartin

  • 1Area de Bioquímica y Biología Molecular, Universidad de la Rioja, Logroño, Spain.

The Journal of Antimicrobial Chemotherapy
|March 1, 1994
PubMed
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Vancomycin-resistant Enterococcus strains, including a novel vanA-mediated resistance in Enterococcus durans, were found in Spanish sewage. This highlights the environmental presence of antibiotic resistance genes.

Area of Science:

  • Environmental microbiology
  • Antimicrobial resistance research
  • Molecular biology

Background:

  • Vancomycin resistance in Enterococcus species is a significant public health concern.
  • Enterococcus species are common inhabitants of the human gut and can be found in wastewater.
  • The vanA gene confers high-level vancomycin resistance.

Purpose of the Study:

  • To investigate the presence of vancomycin-resistant Enterococcus (VRE) in sewage systems.
  • To characterize the mechanism of vancomycin resistance in isolated strains.
  • To report the first confirmed instance of vanA-mediated vancomycin resistance in Enterococcus durans.

Main Methods:

  • Collection and analysis of sewage samples from Logroño, Spain.
  • Isolation and identification of Enterococcus strains.

Related Experiment Videos

  • Detection of VanA protein via immunoblotting.
  • Confirmation of the vanA gene using DNA-DNA hybridization and in transconjugants.

    • Main Results:

    • Three high-level vancomycin-resistant Enterococcus strains were identified in sewage samples.
    • Two strains were Enterococcus faecium, and one was Enterococcus durans.
    • The vanA gene and VanA protein were confirmed as the mechanism of resistance, marking the first report for E. durans.

    Conclusions:

    • Vancomycin-resistant Enterococcus strains, mediated by the vanA gene, are present in the general sewage system of Logroño, Spain.
    • The detection of vanA-mediated resistance in Enterococcus durans represents a novel finding.
    • Wastewater environments can serve as reservoirs for antibiotic-resistant bacteria and genes.