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Viral mutation affecting bacteriophage phi 1 development in Bacillus subtilis 168

C W Rettenmier, H E Hemphill

    Journal of Virology
    |April 1, 1975
    PubMed
    Summary

    Bacillus subtilis bacteriophage phi 1m exhibits abortive infection in B. subtilis 168 at 37°C, halting nucleic acid synthesis. This phage-host interaction is temperature-dependent and influenced by infection multiplicity.

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    Area of Science:

    • Microbiology
    • Virology
    • Bacteriology

    Background:

    • Bacillus subtilis bacteriophage phi 1m is a host-range variant derived from virulent bacteriophage phi 1.
    • Bacteriophage phi 1m demonstrates an inability to form plaques on B. subtilis 168 lawns at 37°C, despite successful adsorption, penetration, and bacterial lysis.

    Purpose of the Study:

    • To investigate the mechanism of abortive infection of Bacillus subtilis 168 by bacteriophage phi 1m at 37°C.
    • To elucidate the role of infection multiplicity and host cell temperature in the phi 1m-B. subtilis interaction.

    Main Methods:

    • Comparative plaque assays on B. subtilis 168 at 37°C.
    • Liquid culture experiments to assess phage development at varying multiplicities of infection.
    • Analysis of host and phage nucleic acid synthesis under nonpermissive conditions.

    Main Results:

    • Bacteriophage phi 1m infection of B. subtilis 168 at 37°C resulted in abortive development, particularly at high multiplicities of infection.
    • Permissive infection was observed in specific B. subtilis 168 mutants and in wild-type cells grown above 47°C.
    • Under nonpermissive conditions, transcription and DNA synthesis ceased approximately 20 minutes post-infection.

    Conclusions:

    • The abortive infection of B. subtilis 168 by phi 1m at 37°C is a complex phenomenon influenced by temperature and infection dose.
    • Host cell physiology, potentially related to nucleic acid metabolism, plays a critical role in determining the outcome of bacteriophage infection.

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